We introduce a method of in vitro recombination or “DNA shuffling” to generate libraries of evolved enzymes. The approach relies on the ordering, trimming, and joining of randomly cleaved parental DNA ...
1| Combine the following components of the master mix on ice and mix thoroughly: 10× KLA reaction buffer (pH 9.2): 500 mM Tris base, 160 mM (NH 4) 2 SO 4, 25 mM MgCl 2, 1% Tween 20. Note, the pH is ...
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