Restriction mapping is a method used to map an unknown segment of DNA by breaking it into pieces and then identifying the locations of the breakpoints. This method relies upon the use of proteins ...

Modular cloning and expression of complex genetic assemblies are becoming widely popular. Recent applications based on site-specific and homologous recombination have substantially improved the ...

Key to Invitrogen's Gateway technology is the "entry clone," which contains a fragment of interest in a recombinase-ready plasmid. Researchers can quickly and efficiently move between expression ...