Before setting a gating strategy, it is vital to know the properties of cells to be analyzed, such as cell size, the relative expression of markers and whether artifacts are likely to have been ...

Differences in how users gate populations within experiments are major sources of variability in flow cytometry data analysis. Incorporating automated image analysis can substantially reduce user bias ...

A group from the Shanghai Jiao Tong University School of Medicine has outlined a protocol for the tissue-resident myeloid populations in mouse tissues using flow cytometry. The protocol builds on ...

The quality of reference controls is critical for generating accurate compensation or unmixing matrices and correctly interpreting conventional or spectral flow cytometry data, respectively. Follow ...

Technological change is often driven by the needs of people. Flow cytometry is no different. The designs of instruments and processing techniques are evolving to meet the needs of researchers and drug ...

Immunophenotyping is the identification and quantitation of heterogeneous populations of cells by multi-parameter flow cytometry using a panel of fluorescently-labelled antibodies that recognize ...