To increase the levels of pulmonary gene transfer by nonviral vectors, we have adopted electroporation protocols for use in the lung. A volume of 100–200 μl of purified plasmid DNA suspended in saline ...

When a cell is able to take up free DNA or RNA, it is termed “competent”. Some cell types or microbial species, such as Bacillus subtilis, 11 are naturally competent and they can be induced to uptake ...

Cell therapy is an emerging novel treatment option for an array of diseases, ranging from genetic disorders to cancer. As the cell therapy field expands, there is a growing demand for safe and ...

Electroporation — the use of high-voltage electric pulses to introduce DNA/RNA/proteins into cells — has made cellular engineering and gene expression easier than ever imagined. One version of this ...

Unleash the power of electroporation with the Invitrogen Neon NxT Electroporation System. Join us for an insightful webinar where we demystify the basics of electroporation and guide you on how to ...

Graduate students Mingde ‘Jack’ Zheng and Joseph Sherba have developed a novel, microfluidic platform for monitoring electroporation and molecular delivery at the single cell-level as part of a NSF ...

Using an electric current to deliver the CRISPR/Cas9 system, in order to engineer genetic changes in laboratory mice, is highly efficient and significantly improves the system's throughput over the ...

Naked mRNA administration in the testes rescues sperm function, demonstrating a potential therapeutic approach for genetic male infertility.

Pulsed electric field, also known as electroporation, is an exciting advancement in electrosurgery and life sciences that enables the development of numerous novel treatments. As a result, this ...

3-dimensional rendering of a cell passing through the electroporation zone while a pulse is being applied. The electric field lines which normally do not penetrate an insulating cell are able to ...